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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Direct expression of active human tissue inhibitors of metalloproteinases by periplasmic secretion in Escherichia coli

Fig. 2

Periplasmic production of (N-)TIMPs and expression condition optimization. a Reducing SDS-PAGE of purified (N-)TIMPs stained with Coomassie blue. Red arrows indicate the target bands. b Effect of periplasmic folding modulators (DsbA and/or DsbC) on expression efficiencies of (N-)TIMPs analyzed by Western blotting using anti-6×His antibody. 1P indicates DsbA and DsbC were under one P BAD promoter and 2P represents DsbA and DsbC were under two separated P BAD promoters. Red arrows indicate the target bands. c Effect of BL21(DE3) on reducing unwanted truncations analyzed by Western blotting. Same amounts of cells were used in b and c

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