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Table 3 Purified PduP-E yields and activity

From: Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli

Sample Induction conditions Total yield (µg/L of culture) Activity (% growth inhibition after 6 h)a
PduP-E from Ec3087 0.1 mM Rha 200 72 ± 3
PduP-E from Ec3087 0.1 mM Rha + 0.5 mM IPTG 270 70 ± 5
EBB buffer control N/A N/A 34 ± 6
E after Factor Xa cleavage 0.1 mM Rha + 0.5 mM IPTG 200 49 ± 6
Factor Xa control N/A N/A 28 ± 2
  1. aActivity is presented as the percent that B. licheniformis growth is inhibited by 0.5 µM of E in culture compared to normal growth after 6 h based on OD600