Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli

Table 3 Purified PduP-E yields and activity

Sample	Induction conditions	Total yield (µg/L of culture)	Activity (% growth inhibition after 6 h)^a
PduP-E from Ec3087	0.1 mM Rha	200	72 ± 3
PduP-E from Ec3087	0.1 mM Rha + 0.5 mM IPTG	270	70 ± 5
EBB buffer control	N/A	N/A	34 ± 6
E after Factor Xa cleavage	0.1 mM Rha + 0.5 mM IPTG	200	49 ± 6
Factor Xa control	N/A	N/A	28 ± 2

^aActivity is presented as the percent that B. licheniformis growth is inhibited by 0.5 µM of E in culture compared to normal growth after 6 h based on OD₆₀₀

ISSN: 1475-2859