Strain | Induction conditions | Total PduP-tagged protein in µg (% in total lysate)a | Total BMC yield (mg)b | PduP-tagged protein/BMCs (µg/mg) | ||
---|---|---|---|---|---|---|
Lysate | Soluble fraction | Isolated BMCs | ||||
Ec0087 | 0.2 mM Rha | 1300 (100%) | 75 (5.8%) | 0.063 (0.0049%) | 0.062 | 1.0 |
Ec3087 | 0.1 mM Rha + 0.5 mM IPTG | 1300 (100%) | 380 (28%) | 18 (1.4%) | 1.4 | 14 |
Ec3087 | 0.2 mM Rha + 0.5 mM IPTG | 1700 (100%) | 170 (10%) | 8.7 (0.52%) | 0.74 | 12 |
Ec3087 | 0.5 mM Rha + 0.5 mM IPTG | 1100 (100%) | 120 (11%) | 12 (1.1%) | 0.94 | 13 |
Ec3090 | 0.2 mM Rha + 0.5 mM IPTG | 29,000 (100%)c | 22,000 (75%)c | 73 (0.25%)c | 0.88 | 39a |