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Table 2 Amounts of PduP-tagged protein isolated with BMCs

From: Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli

Strain Induction conditions Total PduP-tagged protein in µg (% in total lysate)a Total BMC yield (mg)b PduP-tagged protein/BMCs (µg/mg)
Lysate Soluble fraction Isolated BMCs
Ec0087 0.2 mM Rha 1300 (100%) 75 (5.8%) 0.063 (0.0049%) 0.062 1.0
Ec3087 0.1 mM Rha + 0.5 mM IPTG 1300 (100%) 380 (28%) 18 (1.4%) 1.4 14
Ec3087 0.2 mM Rha + 0.5 mM IPTG 1700 (100%) 170 (10%) 8.7 (0.52%) 0.74 12
Ec3087 0.5 mM Rha + 0.5 mM IPTG 1100 (100%) 120 (11%) 12 (1.1%) 0.94 13
Ec3090 0.2 mM Rha + 0.5 mM IPTG 29,000 (100%)c 22,000 (75%)c 73 (0.25%)c 0.88 39a
  1. aAmounts of PduP-tagged proteins were determined by quantitative Western blots using anti-His6 (see Additional file 1: Figure S9 for raw data)
  2. bTotal protein content was determined by Bradford assay using BSA as a standard
  3. cmg of PduP-mCherry was estimated based on a PduP-E standard curve, assuming anti-His6 detects an equal number of PduP-E and PduP-mCherry molecules [i.e., 1 ng of PduP-E (14.8 kDa) detected is equivalent to 2.1 ng of PduP-mCherry (31.2 kDa) detected]