Skip to main content

Table 2 Amounts of PduP-tagged protein isolated with BMCs

From: Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli

Strain

Induction conditions

Total PduP-tagged protein in µg (% in total lysate)a

Total BMC yield (mg)b

PduP-tagged protein/BMCs (µg/mg)

Lysate

Soluble fraction

Isolated BMCs

Ec0087

0.2 mM Rha

1300 (100%)

75 (5.8%)

0.063 (0.0049%)

0.062

1.0

Ec3087

0.1 mM Rha + 0.5 mM IPTG

1300 (100%)

380 (28%)

18 (1.4%)

1.4

14

Ec3087

0.2 mM Rha + 0.5 mM IPTG

1700 (100%)

170 (10%)

8.7 (0.52%)

0.74

12

Ec3087

0.5 mM Rha + 0.5 mM IPTG

1100 (100%)

120 (11%)

12 (1.1%)

0.94

13

Ec3090

0.2 mM Rha + 0.5 mM IPTG

29,000 (100%)c

22,000 (75%)c

73 (0.25%)c

0.88

39a

  1. aAmounts of PduP-tagged proteins were determined by quantitative Western blots using anti-His6 (see Additional file 1: Figure S9 for raw data)
  2. bTotal protein content was determined by Bradford assay using BSA as a standard
  3. cmg of PduP-mCherry was estimated based on a PduP-E standard curve, assuming anti-His6 detects an equal number of PduP-E and PduP-mCherry molecules [i.e., 1 ng of PduP-E (14.8 kDa) detected is equivalent to 2.1 ng of PduP-mCherry (31.2 kDa) detected]