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Table 1 Plasmids and bacterial strains used in this study

From: Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli

Plasmid/strain Description Reference
Plasmids
 pD444-SR Expression vector, IPTG-inducible T5 promoter, lacI, ori pUC, amp (Amr) ATUM
 pD861 Expression vector, rhamnose-inducible pRha promoter, ori pUC, kan (Kmr) ATUM
 pMCY29 pD444-SR-derived vector expressing eutSMNLK This study
 pMCY30 pD444-SR-derived vector expressing pduABJKNU This study
 pMCY31 pD444-SR-derived vector expressing eutS This study
 pMCY85 pD861-derived vector expressing the gene for EutC1–19-X-E-T-His6 fusion This study
 pMCY86 pD861-derived vector expressing the gene for PduD1–20-X-E-T-His6 fusion This study
 pMCY87 pD861-derived vector expressing the gene for PduP1–18-X-E-T-His6 fusion This study
 pMCY90 pD861-derived vector expressing the gene for PduP1–18-X-mCherry-T-His6 fusion This study
 pMCY99 pD444-SR-derived vector expressing mcherry and pduJKNU This study
 pMCY101 pD444-SR-derived vector expressing mcherry This study
Strains
 E. coli BL21 Expression strain for non-T7 protein expression, fhuA2 [lon] ompT gal [dcm] ΔhsdS NEB
 Ec0030 E. coli BL21 harboring pMCY30 This study
 Ec2985 E. coli BL21 harboring pMCY29 and pMCY85 This study
 Ec3185 E. coli BL21 harboring pMCY31 and pMCY85 This study
 Ec3086 E. coli BL21 harboring pMCY30 and pMCY86 This study
 Ec0087 E. coli BL21 harboring pMCY87 This study
 Ec3087 E. coli BL21 harboring pMCY30 and pMCY87 This study
 Ec3090 E. coli BL21 harboring pMCY30 and pMCY90 This study
 Ec9987 E. coli BL21 harboring pMCY99 and pMCY87 This study
 Ec10187 E. coli BL21 harboring pMCY101 and pMCY87 This study
  1. X factor Xa protease cleavage site, T thrombin protease cleavage site, E lysis protein E