Table 1 Plasmids and bacterial strains used in this study
Plasmid/strain | Description | Reference |
|---|---|---|
Plasmids | ||
pD444-SR | Expression vector, IPTG-inducible T5 promoter, lacI, ori pUC, amp (Amr) | ATUM |
pD861 | Expression vector, rhamnose-inducible pRha promoter, ori pUC, kan (Kmr) | ATUM |
pMCY29 | pD444-SR-derived vector expressing eutSMNLK | This study |
pMCY30 | pD444-SR-derived vector expressing pduABJKNU | This study |
pMCY31 | pD444-SR-derived vector expressing eutS | This study |
pMCY85 | pD861-derived vector expressing the gene for EutC1–19-X-E-T-His6 fusion | This study |
pMCY86 | pD861-derived vector expressing the gene for PduD1–20-X-E-T-His6 fusion | This study |
pMCY87 | pD861-derived vector expressing the gene for PduP1–18-X-E-T-His6 fusion | This study |
pMCY90 | pD861-derived vector expressing the gene for PduP1–18-X-mCherry-T-His6 fusion | This study |
pMCY99 | pD444-SR-derived vector expressing mcherry and pduJKNU | This study |
pMCY101 | pD444-SR-derived vector expressing mcherry | This study |
Strains | ||
E. coli BL21 | Expression strain for non-T7 protein expression, fhuA2 [lon] ompT gal [dcm] ΔhsdS | NEB |
Ec0030 | E. coli BL21 harboring pMCY30 | This study |
Ec2985 | E. coli BL21 harboring pMCY29 and pMCY85 | This study |
Ec3185 | E. coli BL21 harboring pMCY31 and pMCY85 | This study |
Ec3086 | E. coli BL21 harboring pMCY30 and pMCY86 | This study |
Ec0087 | E. coli BL21 harboring pMCY87 | This study |
Ec3087 | E. coli BL21 harboring pMCY30 and pMCY87 | This study |
Ec3090 | E. coli BL21 harboring pMCY30 and pMCY90 | This study |
Ec9987 | E. coli BL21 harboring pMCY99 and pMCY87 | This study |
Ec10187 | E. coli BL21 harboring pMCY101 and pMCY87 | This study |