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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli

Fig. 3

Co-expression of PduP-E with mCherry (Ec10187) versus with the Pdu BMC system (Ec3087). a Genetic scheme for the different strains. Black circles represent ribosome binding/re-initiation sites. b Growth curves for each strain under different induction conditions. Open shapes induction with 0.1 mM rhamnose only at t = 0; closed shapes co-induction with 0.5 mM IPTG at t = −3 h and 0.1 mM rhamnose at t = 0. Error bars represent data from 3 replicates. c SDS-PAGE and anti-His6 Western blot at 4 h post-rhamnose induction from 1 representative growth. The equivalent of 50 and 2 µL of original culture were analyzed by SDS-PAGE and Western blot, respectively. Sizes of molecular weight standards in kDa are shown on the left. d Relative Western blot intensities compared to Ec3087, Rha only. Gray bars rhamnose only induction; striped bars IPTG and rhamnose co-induction. Error bars represent data from 3 replicates from the different growths in b (see Additional file 1: Figure S6 for raw data)

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