Fig. 2

Growth and PduP-E expression in Ec3087 and Ec0087 under different induction conditions. Ec0087 induced with rhamnose only (open circle); Ec3087 induced with rhamnose only (open square); and Ec3087 co-induced with rhamnose and IPTG (filled triangle). IPTG was added at t = −3 h to 0.5 mM. Rhamnose was added at t = 0 to 0.1 mM in a, d, g, to 0.2 mM in b, e, h, and to 0.5 mM in c, f, i. Error bars represent data from 3 biological replicates. a–c Growth of cells. d–f Amounts of PduP-E expressed per L of culture determined by quantitative Western blot (see Additional file 1: Figure S5 for raw data). g–i Amounts of PduP-E expressed per L of culture normalized to OD₆₀₀