Fig. 1

Growth and E expression in different BMC systems. a Genetic scheme for the different BMC systems. Black circles represent ribosome binding/re-initiation sites. b Growth curves for each strain under different induction conditions. Induction with 0.5 mM IPTG only at t = −3 h (open circle); induction with 0.1 mM rhamnose only at t = 0 (open square); and co-induction with both IPTG and rhamnose (filled triangle). Error bars represent data from 3 replicates. c SDS-PAGE and anti-His₆ Western blot at 4 h post-rhamnose induction from 1 representative growth. The equivalent of 50 µL and 2 µL of original culture were analyzed by SDS-PAGE and Western blot, respectively. Left red arrows EutL (top), EutS/M/N (bottom). Right red arrows PduB (top), PduB′ (middle), and PduA/J (bottom). Sizes of molecular weight standards in kDa are shown on the left. d Relative Western blot intensities compared to Ec2985, Rha only. Gray bars rhamnose only induction; striped bars IPTG and rhamnose co-induction. Error bars represent data from 3 replicates from the different growths in b (see Additional file 1: Figure S2 for raw data)