Table 1 Strains and plasmids used in this study
Name | Description | Reference |
|---|---|---|
Plasmids | ||
pYX212 | 2 μm, AmpR, URA3, TPIp, pYX212t | R&D systems |
pAlkane78 | pYX212-(TPIp-Mdb5-FBA1t-CYC1t-MdCPR-TDH3p‐tHXT7P-CYP4G1‐pYX212t) | This study |
pAlkane8 | pYX212-(TPIp-Mdb5-FBA1t-CYC1t-MdCPR-TDH3p‐tHXT7P-CYP4G2‐pYX212t) | This study |
pAlkane71 | pYX212-(eTDH3p‐CER1-Syn27t-pYX212t) | This study |
pAlkane67 | pYX212-(eTDH3p‐SeADO‐pYX212t) | [17] |
pAlkane83 | pYX212-(eTDH3p‐CwADO‐pYX212t) | This study |
pAlkane84 | pYX212-(eTDH3p‐TeADO‐pYX212t) | This study |
pAlkane85 | pYX212-(eTDH3p‐CyADO‐pYX212t) | This study |
pAlkane86 | pYX212-(CYC1t-CwADO-Gal10p-Gal1p-SeADO-pYX212t) | This study |
Strains | ||
DH5α | F− (80d lacZ M15) (lacZYA-argF) U169 hsdR17(r− m+) recA1 endA1 relA1 deoR96 | |
YJZ60 | MATa MAL2-8c SUC2 his3Δ1ura3-52 hfd1Δpox1Δ Gal80Δ:: SeFNR + SeFd adh5Δ::(TPIp-MmCAR-FBA1t) + (PGK1p-EcFNR-CYC1t) + (TEF1p-EcFD-TDH2t) + (tHXT7p-npgA-ADH5t) | [17] |
Con | YJZ60 strain harboring pYX212 | This study |
CYP4G1 | YJZ60 strain harboring pAlkane78 | This study |
CYP4G2 | YJZ60 strain harboring pAlkane8 | This study |
CER1 | YJZ60 strain harboring pAlkane71 | This study |
SeADO | YJZ60 strain harboring pAlkane67 | This study |
CwADO | YJZ60 strain harboring pAlkane83 | This study |
TeADO | YJZ60 strain harboring pAlkane84 | This study |
CyADO | YJZ60 strain harboring pAlkane85 | This study |
CSADO | YJZ60 strain harboring pAlkane86 | This study |