Table 1 Yeast strains used in the study
ID | Species | Information | Source |
|---|---|---|---|
Brewing strains | |||
P1 | S. cerevisiae | VTT-A81062. Maltotriose fermentation, POF+ | VTT culture collection |
P2 | S. cerevisiae | WLP099. No maltotriose fermentation, POF− | White Labs Inc |
P3 | S. eubayanus | VTT-C12902. No maltotriose fermentation, POF+ | VTT culture collection |
H1 | Hybrid | P1 × P3 interspecific hybrid (VTT-A15225) | Created in this study |
H2 | Hybrid | P2 × P3 interspecific hybrid | Created in this study |
H3 | Hybrid | P1 × P2 intraspecific hybrid | Created in this study |
T1 | Hybrid | H1 × P2 interspecific triple hybrid | Created in this study |
T2 | Hybrid | Meiotic segregant of T1 | Created in this study |
Laboratory strains | |||
WT | S. cerevisiae | BY4741 MAT a; his3∆1; leu2∆0; met15∆0; ura3∆0 | EUROSCARF (Y00000) |
ole1∆ | S. cerevisiae | BY4741 MAT a; his3∆1; leu2∆0; met15∆0; ura3∆0; ole1-m2:kanMX | EUROSCARF (Y40963) |
erg4∆ | S. cerevisiae | BY4741 MAT a; his3∆1; leu2∆0; met15∆0; ura3∆0; YGL012w::kanMX4 | EUROSCARF (Y04380) |