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Fig. 9 | Microbial Cell Factories

Fig. 9

From: Inheritance of brewing-relevant phenotypes in constructed Saccharomyces cerevisiae × Saccharomyces eubayanus hybrids

Fig. 9

The karyotype stability of Hybrid T2 was assessed after 10 successive batch cultures. a The DNA content of Hybrid T2, the 4 isolates obtained after 10 cultures in Media 1, and the 4 isolates obtained after 10 cultures in Media 2 as determined by flow cytometry. The displayed values are the geometric mean and standard deviation of the 1 N peak. All 8 isolates showed a significant decrease in DNA content (p < 0.05 as determined by a two-sample Kolmogorov–Smirnov test). b PFGE separation of chromosomes from Hybrid T2 and 8 isolates obtained after 10 cultures in Media 1 and 2. Lanes 1, 7 and 13 chromosome marker strain YNN295, lanes 2 and 8 Hybrid T2, lanes 3–6 the 4 isolates obtained after 10 cultures in Media 1, and lanes 9–12 the 4 isolates obtained after 10 cultures in Media 2. The red box depicts an area where differences in chromosomes were observed in the isolates compared to Hybrid T2. Reference chromosomes are identified on the right: chromosomes VII and XV are not resolved; chromosome II travels immediately above chromosome XIV

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Microbial Cell Factories

ISSN: 1475-2859

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