Fig. 1

Overview of the 8 brewing yeast strains created and used in the study. First, mutants of parent strains P1–P3 carrying selection markers were selected. Hybrids H1–H3 were then obtained by rare mating parent strains P1–P3. Hybrid T1, containing DNA from all three parent strains, was obtained by sporulating the allotetraploid Hybrid H1 and rare mating dissected spores with parent P2. The POF− segregant Hybrid T2 was obtained by sporulating Hybrid T1 and screening spore clones for the absence of 4-vinyl guaiacol production. For more information see the “Methods” and Table 1. Scer: Saccharomyces cerevisiae, Seub: Saccharomyces eubayanus