Skip to main content

Table 2 The main strains and plasmids used in this study

From: Cell surface engineering of Bacillus subtilis improves production yields of heterologously expressed α-amylases

Strain or plasmid

Genotype or properties

Reference or source

B. subtilis

 DB104

his nprE aprE

[26]

 JMM8900

DB104 thrC::spaRK, Emr

This study

 JMM8901

DB104 thrC::spaRK, Emr, amyE::Spcr

This study

 JMM8901-dacA

DB104 thrC::spaRK, Emr, amyE::Spcr, dacA::Kmr

This study

 JMM8901-dltA

DB104 thrC::spaRK, Emr, amyE::Spcr, dltA::Kmr

This study

 JMM8901-tagO

DB104 thrC::spaRK, Emr, amyE::Spcr, tagO::Kmr

This study

 JMM8901-tuaA

DB104 thrC::spaRK, Emr, amyE::Spcr, tuaA::Kmr

This study

 JMM8901-pssA

DB104 thrC::spaRK, Emr, amyE::Spcr, pssA::Kmr

This study

 JMM8901-clsA

DB104 thrC::spaRK, Emr, amyE::Spcr, clsA::Kmr

This study

 JMM8901-pssA +clsA

DB104 thrC::spaRK, Emr, amyE::Spcr, pssA::Tetr, clsA::Kmr

This study

E.coli

 MC1061

F-, araD139, Δ(ara-leu)7696, Δ(lac)X74, galU,

galK, hsdR2, mcrA, mcrB1, rspL

[36]

Plasmids

 pNZ8901

PspaSpn repC repA, Cmr

[31]

 pNZ8901-Amy#707

pNZ8901 carrying amy#707

This study

 pNZ8901-AmyTS-23

pNZ8901 carrying amyTS-23

This study

 pNZ8901-AmyBs

pNZ8901 carrying amyBs

This study

 pNZ8901-AmyBm

pNZ8901 carrying amyBm

This study

 pNZ8901-AmyK38

pNZ8901 carrying amyK38

This study

 pUC21

Ampr lacZ

[37]

 pUC21_DacAKO

pUC21_dacAUp_Kmr _dacADown

This study

 pUC21_DltAKO

pUC21_dltAUp_Kmr _dltADown

This study

 pUC21_TagOKO

pUC21_tagOUp_Kmr _tagODown

This study

 pUC21_TuaAKO

pUC21_tuaAUp_Kmr _tuaADown

This study

 pUC21_PssAKO

pUC21_pssAUp_Kmr _pssADown

This study

 pUC21_ClsAKO

pUC21_clsAUp_Kmr _clsADown

This study

 pUC21_PssAKO(Tetr)

pUC21_pssAUp_Tetr _pssADown

This study