Table 2 Primers used in this study
From: Intramembrane protease RasP boosts protein production in Bacillus
Primer name | 5′ → 3′ nucleotide sequence |
|---|---|
CI2.rev | CTTCAACGCTAACTTTGAG |
rasP.P0 | GCTCTTCAAGGCGAACAGG |
rasP.P1 | CGCCTCATCATTACGGCATC |
rasP.P2 | CGACCTGCAGGCATGCAAGCTACCACCTTATGTGAGTATTGAATTGAC |
rasP.P3 | CGAGCTCGAATTCACTGGCCGTCGGGATACGTCAATTCAATACTCACATAAGGTGGTACGAAAAGTAAATCAATCAGAGGTGC |
rasP.P4 | GATCGTACGGCGCAACG |
tepA.P0 | CGCACGGGCACGATG |
tepA.P1 | CTGTCCGTTCCAGTGTACGG |
tepA.P2 | CGACCTGCAGGCATGCAAGCTCTCGCTTTCATCCTTTCCG |
tepA.P3 | CGAGCTCGAATTCACTGGCCGTCGCAAAGAGAAACTCGGAAAGGATGAAAGCGAGTTCTTTATACCGTGATGCCTCAG |
tepA.P4 | GGTCTGTCATTCAATTTAGACTCCAG |
pHT315CPEC.fw | CTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC |
pHT315CPEC.rev | CACTGTTTTTAGTCTGTTTCAAAACAGTAG |
kanaCPEC.fw | CTACTGTTTTGAAACAGACTAAAAACAGTGGCTCCGTCGATACTATGTTATACG |
kanaCPEC.rev | TAACTTTCCAAATTTAAAAAAGCGACTCATAGGCTTTTTAGACATCTAAATCTAGGTAC |
pHT315rasP.fw | GTGAGTATTGAATTGACGTATCCCCGCCCGGGTACCGAGCTCTTAATTG |
pHT315rasP.rev | GCGAAGAAATGAGACAAAGCTTGACGGCTTGGCGTAATCATGGTCATAGC TG |
rasP.fw | CGGGGATACGTCAATTCAATACTCAC |
rasP.rev | CGTCAAGCTTTGTCTCATTTCTTCGC |