Table 1 Primers used in the construction of plasmids
From: Development of an efficient technique for gene deletion and allelic exchange in Geobacillus spp.
Oligonucleotide name | Sequence |
|---|---|
Bgl_F | atgcctgcaggtcgactctagaggatccccacgggccagtttgttgaaga |
Bgl_R | accatgattacgaattcgagctcggtacccttagctgcgatacgctccgc |
Pdu-start_F | ggcgtctaacaggcgcgtagagacatggttcgagaagcattagg |
Pdu-start_R | atattcccccgacttaaagacgctaaaatttctgtattggttgc |
Pdu-5′_F | gcatcaggcgccattcgccattcaccagtcatccatattgctttag |
Pdu-5′_R | cccggggcggccgcagctagcacgtggacctccctctacta |
Pdu-3′_F | gctagctgcggccgccccgggtctttgggagggaatgttatgag |
Pdu-3′_R | cttcccaacagttgcgcagcctcacggcctgtttgtgagccaac |
LdhF | aaaaaagctagctcgcggagcggggaattaaaaag |
LdhR | aaaaaacccgggcgctgtctgtcatcctttcc |
Test_F | gaatgctttagaatatggag |
Test_R | gagcttctagaaaatacag |