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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Development of an efficient technique for gene deletion and allelic exchange in Geobacillus spp.

Fig. 2

Colony PCR to confirm the first crossover event. Following re-streaking, five colonies were selected from agar with kanamycin and X-Glu, boiled in 20 µl milliQ water and PCR amplified with Bgl_F and Bgl_R. Following a double-crossover, the plasmid bgl gene would not be present. Lane 1 Biolabs 1 Kb ladder, lane 2 the 1.1 kb control bgl PCR product from unintegrated TM242 pUCG3.8Bgl-pdu, lanes 37 no PCR product from colonies selected on kanamycin X-Glu plates

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