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TableĀ 2 Strains

From: Production of (S)-2-aminobutyric acid and (S)-2-aminobutanol in Saccharomyces cerevisiae

Strains

Description

Selection marker

Replication origin/integration site

EVST20590

Contains expression cassettes of ScCHA1 and ScGDH1ā€²

URA3

ARS/CEN

EVST20591

Contains expression cassettes of ScCHA1 and ScGDH3ā€²

URA3

ARS/CEN

EVST20592

Contains expression cassettes of ScCHA1 and EcGDHā€™

URA3

ARS/CEN

EVST20879

Contains expression cassettes of ScCHA1 and BcLeuDH

URA3

ARS/CEN

EVST20880

Contains expression cassettes of ScCHA1 and BfLeuDH

URA3

ARS/CEN

EVST20881

Contains expression cassettes of ScGDH1ā€² and EcILVa

URA3

ARS/CEN

EVST20882

Contains expression cassettes of ScGDH3ā€² and EcILVa

URA3

ARS/CEN

EVST20883

Contains expression cassettes of EcGDHā€™ and EcILVa

URA3

ARS/CEN

EVST20884

Contains expression cassettes of EcILVa and BcLeuDH

URA3

ARS/CEN

EVST20885

Contains expression cassettes of EcILVa and BfLeuDH

URA3

ARS/CEN

EVST21351

Contains expression cassettes of ScCHA1 and SfValDH

URA3

ARS/CEN

EVST21352

Contains expression cassettes of EcILVa and SfValDH

URA3

ARS/CEN

EVST21478

Contains expression cassettes of ScCHA1, EcGDHā€™, NiCAR, EcALR, and BsSFP

URA3

ARS/CEN

EVST21479

Contains expression cassettes of ScCHA1, EcGDHā€™, NfCAR, EcALR, and BsSFP

URA3

ARS/CEN

EVST21542

Control strain with empty entry vectors

URA3

ARS/CEN

EVST22605

Contains expression cassettes of BsILVa and EcGDHā€™

URA3

ARS/CEN

EVST22606

Contains expression cassettes of ScILV1 and EcGDHā€™

URA3

ARS/CEN

EVST22608

Contains expression cassettes of SlTD and EcGDHā€™

URA3

ARS/CEN

EVST22609

Contains expression cassette of ScHOM3-R2

LEU2

2 micron

EVST22610

Contains expression cassettes of BsILVa, EcGDHā€™, and ScHOM3-R2

URA3 (BsILVa, EcGDHā€™), LEU2 (ScHOM3-R2)

ARS/CEN (BsILVa, EcGDHā€™)

2Ā micron (ScHOM3-R2)

EVST22615

Contains expression cassettes of EcILVa, EcGDHā€™, MsCAR, MsPPTase, and EcALR

URA3

ARS/CEN

EVST22837

Contains expression cassette of SlTD and two EcGDHā€™ expression cassettes

URA3, HIS3

ARS/CEN both

EVST22857

Deletion of GLY1 ORF

ā€“

ā€“

EVST23406

Deletion of GLY1 ORF

Contains expression cassettes of BsILVa and EcGDHā€™

URA3

ARS/CEN

EVST23407

Deletion of GLY1 ORF

Contains expression cassettes of SlTD and EcGDHā€™

URA3

ARS/CEN

EVST24185

Contains expression cassettes of BsILVa, EcGDHā€™, MmCAR, EcALR, and BsSFP

URA3

ARS/CEN

EVST25556

Control strain with empty plasmid

LEU2

2 micron

EVST25557

Contains expression cassettes of BsILVa, EcGDHā€™, and empty plasmid

URA3 (BsILVa, EcGDHā€™), LEU2

ARS/CEN (BsILVa, EcGDHā€™)

2 micron (empty plasmid)

EVST25635

Contains expression cassettes of SlTD, EcGDHā€™, and empty plasmid

URA3 (BsILVa, EcGDHā€™), HIS3

ARS/CEN both

EVST27022

Contains expression cassettes of BsILVa, EcGDHā€™, MmCAR, EcALR, and BsSFP

URA3

integrated into Chromosome XI-2