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Table 2 Strains

From: Production of (S)-2-aminobutyric acid and (S)-2-aminobutanol in Saccharomyces cerevisiae

Strains Description Selection marker Replication origin/integration site
EVST20590 Contains expression cassettes of ScCHA1 and ScGDH1′ URA3 ARS/CEN
EVST20591 Contains expression cassettes of ScCHA1 and ScGDH3′ URA3 ARS/CEN
EVST20592 Contains expression cassettes of ScCHA1 and EcGDH’ URA3 ARS/CEN
EVST20879 Contains expression cassettes of ScCHA1 and BcLeuDH URA3 ARS/CEN
EVST20880 Contains expression cassettes of ScCHA1 and BfLeuDH URA3 ARS/CEN
EVST20881 Contains expression cassettes of ScGDH1′ and EcILVa URA3 ARS/CEN
EVST20882 Contains expression cassettes of ScGDH3′ and EcILVa URA3 ARS/CEN
EVST20883 Contains expression cassettes of EcGDH’ and EcILVa URA3 ARS/CEN
EVST20884 Contains expression cassettes of EcILVa and BcLeuDH URA3 ARS/CEN
EVST20885 Contains expression cassettes of EcILVa and BfLeuDH URA3 ARS/CEN
EVST21351 Contains expression cassettes of ScCHA1 and SfValDH URA3 ARS/CEN
EVST21352 Contains expression cassettes of EcILVa and SfValDH URA3 ARS/CEN
EVST21478 Contains expression cassettes of ScCHA1, EcGDH’, NiCAR, EcALR, and BsSFP URA3 ARS/CEN
EVST21479 Contains expression cassettes of ScCHA1, EcGDH’, NfCAR, EcALR, and BsSFP URA3 ARS/CEN
EVST21542 Control strain with empty entry vectors URA3 ARS/CEN
EVST22605 Contains expression cassettes of BsILVa and EcGDH’ URA3 ARS/CEN
EVST22606 Contains expression cassettes of ScILV1 and EcGDH’ URA3 ARS/CEN
EVST22608 Contains expression cassettes of SlTD and EcGDH’ URA3 ARS/CEN
EVST22609 Contains expression cassette of ScHOM3-R2 LEU2 2 micron
EVST22610 Contains expression cassettes of BsILVa, EcGDH’, and ScHOM3-R2 URA3 (BsILVa, EcGDH’), LEU2 (ScHOM3-R2) ARS/CEN (BsILVa, EcGDH’)
2 micron (ScHOM3-R2)
EVST22615 Contains expression cassettes of EcILVa, EcGDH’, MsCAR, MsPPTase, and EcALR URA3 ARS/CEN
EVST22837 Contains expression cassette of SlTD and two EcGDH’ expression cassettes URA3, HIS3 ARS/CEN both
EVST22857 Deletion of GLY1 ORF
EVST23406 Deletion of GLY1 ORF
Contains expression cassettes of BsILVa and EcGDH’
URA3 ARS/CEN
EVST23407 Deletion of GLY1 ORF
Contains expression cassettes of SlTD and EcGDH’
URA3 ARS/CEN
EVST24185 Contains expression cassettes of BsILVa, EcGDH’, MmCAR, EcALR, and BsSFP URA3 ARS/CEN
EVST25556 Control strain with empty plasmid LEU2 2 micron
EVST25557 Contains expression cassettes of BsILVa, EcGDH’, and empty plasmid URA3 (BsILVa, EcGDH’), LEU2 ARS/CEN (BsILVa, EcGDH’)
2 micron (empty plasmid)
EVST25635 Contains expression cassettes of SlTD, EcGDH’, and empty plasmid URA3 (BsILVa, EcGDH’), HIS3 ARS/CEN both
EVST27022 Contains expression cassettes of BsILVa, EcGDH’, MmCAR, EcALR, and BsSFP URA3 integrated into Chromosome XI-2