Fig. 5
From: Production of (S)-2-aminobutyric acid and (S)-2-aminobutanol in Saccharomyces cerevisiae
Intracellular accumulation of 2-ketobutyric acid upon 24 h of growth in selective SC medium. a Two different enzymes for the first step of the (S)-2-aminobutyric acid pathway were expressed (ScCHA1 and EcILVa) in combination with either one of six heterologous enzymes for the second step of the pathway. In case ScCHA1 was expressed or yeasts were not engineered (control), accumulation of 2-ketobutyric acid was below the limit of quantification. b EcGDH’ for the second step of the pathway is expressed in combination with either one of five different threonine deaminating enzymes. Diamonds indicate OD600 after 24 h of growth (all data: mean ± SD, n = 3)