Fig. 10

Intracellular accumulation of 2-ketobutyric acid and (S)-2-aminobutyric acid in S. cerevisiae not expressing GLY1. Wildtype and Δgly1 deletion yeast strains were either engineered for (S)-2-aminobutyric acid production (BsILVa + EcGDH’ or SlTD + EcGDH’), or they contained the empty plasmids (control). Concentrations of (S)-2-aminobutyric acid (orange bars) and 2-ketobutyric acid (blue bars) were analyzed in the yeast pellets upon 24 h of growth in batch culture. Diamonds indicate OD₆₀₀ after 24 h of growth (all data: mean ± SD, n = 3)