Expanding metabolic pathway for de novo biosynthesis of the chiral pharmaceutical intermediate l-pipecolic acid in Escherichia coli

Table 1 Intracellular concentrations of NAD⁺, NADH, NADP⁺, and NADPH in recombinant cells during exponential growth

Strains	Intracellular concentrations (μmol/g_dcw)					NADH/NAD⁺ ratio	NADPH/NADP⁺ ratio
Strains	NAD⁺	NADP⁺	NADH	NADPH	Total	NADH/NAD⁺ ratio	NADPH/NADP⁺ ratio
PA	1.54 ± 0.04	0.14 ± 0.02	0.35 ± 0.02	0.73 ± 0.03	2.76 ± 0.05	0.22 ± 0.02	5.21 ± 0.05
PA-PNTs	1.89 ± 0.07	0.13 ± 0.01	0.39 ± 0.01	0.42 ± 0.03	2.83 ± 0.06	0.20 ± 0.01	3.23 ± 0.03

ISSN: 1475-2859