Fig. 3
From: Heterologous biosynthesis and manipulation of crocetin in Saccharomyces cerevisiae
Combinatorial optimization of CrtZ and CCD from diverse species. a The sketch map of combinations. Each pair of CrtZ and CCD was connected by solid lines in specific color, forming a tested group. Consequently, 36 crocetin producing strains were constructed by screening enzymes from different sources. Eu, E. uredovora; Pa, Pantoea agglomerans; Ss, Sulfolobus solfataricus P2; Ps, Pantoea stewartii; B.SD, Brevundimonas sp. SD212; B.DC, Brevundimonas vesicularis DC263; Hp, Haematococcus pluvialis; Aa, Agrobacterium aurantiacum; As, Alcaligenes sp. PC-1. Determination of crocetin production (b) and zeaxanthin accumulation (c) in each combination by shake flask fermentation. The nine zeaxanthin producing strains carrying different CrtZs without CCDs were used as the blank control. The error bars represent standard deviations calculated from triplicate experiments