Fig. 5
From: Transcriptional reprogramming in yeast using dCas9 and combinatorial gRNA strategies
Triacylglycerol quantification after induced transcriptional regulation. TAG production was engineered in three strains; single gRNAs targeting positions TSS-139 on pDGA1 (pERA-122 in Sc-164) and TSS-381 on pOLE1 (pERA-112 in Sc-162) and multiplexed expression of both gRNAs coupled to dCas9-VPR (pERA-132 in Sc-166). The strains were cultivated in shake flasks for 24 h at 200 rpm, 30 °C. Results are shown as mean ± S.D. from three (n = 3) biological replicate experiments with asterisks indicating significant changes in triacylglycerol content relative to the control strain (*p < 0.05)