Table 2 Plasmids used in this study
Plasmid | Genotype/features | References |
|---|---|---|
pYX212 | pYX212 empty plasmid: 2 μm, ampR, URA3, TPIp, pYX212t | R&D systems |
pAOH3 | pYX212-(TPIp-npgA-FBA1t)-(TDH3p-MmCAR-ADH1t)-(TEF1p-ADH5-pYX212t) | |
pAOH9 | pYX212‐(TPIp‐npgA‐FBA1t)-(TDH3p‐MmCAR‐ADH1t)-(tHXT7p‐ADH5-CYC1t)-(TEF1p‐FaCoAR‐pYX212t) | |
p413 | p413TEF empty plasmid: CEN.ARS, ampR, HIS3, TEF1p, CYC1t | ATCC® 87362 |
pTEF1-FAA1 | p413-TEF1p-FAA1-CYC1t | This study |
pHXT1-FAA1 | p413-HXT1p-FAA1-CYC1t | This study |
pHXT7-FAA1 | p413-HXT7p-FAA1-CYC1t | This study |
pCUP1-FAA1 | p413-CUP1p-FAA1-CYC1t | This study |