Fig. 2

Design of strain producing fatty alcohols from both fatty acyl-CoA and FFAs using dynamically controlled FAA1 expression. a Schematic representation of strain YZFOH2 (YJZ08 pAOH9) expressing FAA1 under different promoters. POX1, HFD1, FAA1 and FAA4 have been deleted in this strain and MmCAR, ADH5 and FaCoAR are overexpressed. b Schematic representation of the plasmids constructed for expression of FAA1. FAA1 is expressed from a CEN.ARS plasmid under the control of promoters CUP1p, TEF1p, HXT1p or HXT7p, represented as pX-FAA1 for pCUP1-FAA1, pTEF1-FAA1, pHXT1-FAA1 or pHXT7-FAA1, respectively. c Schematic representation of the plasmid expressing the enzymes for production of fatty alcohols. npgA, MmCAR, ADH5 and FaCoAR are expressed under strong constitutive promoters from a 2μ plasmid