Fig. 1

Metabolite profiling of YZFOH1 for production of fatty alcohols from FFAs. a Schematic representation of the strain YZFOH1. POX1, HFD1, FAA1 and FAA4 have been deleted in this strain and MmCAR and ADH5 are overexpressed from a 2 µm plasmid (pAOH3). b Total FFAs, fatty alcohols (FOH), glucose and ethanol (EtOH) levels in the culture are represented for YZFOH1. Samples were taken every 3–6 h for 72 h from 3 parallel replicate cultures. c FFA and fatty alcohol titers divided by the culture OD₆₀₀ at each time point