Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris

Table 6 Respiratory quotient (RQ) of fed batch cultures during different cultivation phases

Clone	RQ glycerol batch	RQ MeOH pulse phase	RQ MeOH constant feed
X33 rHSA	0.61 ± 0.03	0.48 ± 0.02	0.52 ± 0.02
Y250 3a rHSA	0.73 ± 0.05	0.60 ± 0.03	0.62 ± 0.02
M250 1a rHSA	0.81 ± 0.08	0.58 ± 0.03	0.64 ± 0.04
M250 3b rHSA	0.59 ± 0.05	0.53 ± 0.02	0.56 ± 0.02

For MeOH phases, value of all pulse phases and constant feed phases were combined. RQ values represent average values ± standard deviation

ISSN: 1475-2859