Skip to main content

Table 2 The final OD600 of ancestral and evolved populations

From: Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris

 

YPM

BMM

YPD

YPDN

BMD

BMDN

YPG

BMG

X-33 wt

2.65 ± 0.09

0.83 ± 0.01

6.46 ± 0.13

4.56 ± 0.22

4.07 ± 0.15

3.09 ± 0.26

6.05 ± 0.16

7.98 ± 0.32

X-33 Y250 1

2.67 ± 0.07

1.26 ± 0.02

7.24 ± 0.13

4.34 ± 0.15

4.20 ± 0.02

2.11 ± 0.09

4.23 ± 0.10

8.77 ± 0.22

X-33 Y250 2

2.94 ± 0.07

1.05 ± 0.02

7.27 ± 0.10

4.80 ± 0.07

4.78 ± 0.10

2.55 ± 0.09

4.01 ± 0.20

8.02 ± 0.17

X-33 Y250 3

2.96 ± 0.02

0.81 ± 0.01

7.69 ± 0.04

4.35 ± 0.04

4.33 ± 0.10

2.35 ± 0.11

4.87 ± 0.13

6.84 ± 0.09

X-33 Y250 4

3.13 ± 0.04

1.01 ± 0.02

7.60 ± 0.15

4.44 ± 0.05

4.31 ± 0.10

2.18 ± 0.08

4.74 ± 0.05

7 47 ± 0.15

X-33 M250 1

2.63 ± 0.0.3

1.42 ± 0.01

7.08 ± 0.20

4.57 ± 0.08

3.70 ± 0.22

4.04 ± 0.02

5.52 ± 0.16

7 26 ± 0.26

X-33 M250 2

2.59 ± 0.08

1.43 ± 0.02

6.86 ± 0.12

4 52 ± 0.04

3.53 ± 0.09

3 18 ± 0.15

5.96 ± 0.13

7.14 ± 0.22

X-33 M250 3

3.33 ± 0.05

1.62 ± 0.01

6.79 ± 0.14

4 59 ± 0.06

4.28 ± 0.12

3.52 ± 0.15

6.10 ± 0.17

6.08 ± 0.07

X-33 M250 4

2.61 ± 0.05

1.43 ± 0.04

7.25 ± 0.05

4 27 ± 0.05

4.28 ± 0.12

3.36 ± 0.17

5.19 ± 0.17

6.23 ± 0.20

  1. Growth tests performed in 24-deep well plates as described in the “Methods” section. X-33 ancestral strain, X-33 Y250a-d populations evolved on YPM medium, X-33 M250a-d populations evolved on BMM medium, YPM YP medium 1% MeOH, BMM buffered minimal medium 1% MeOH, YPD YP medium 2% glucose, YPDN YPD 500 mM NaCl, BMD buffered minimal medium 2% glucose, BMDN BMD 250 mM NaCl, YPG YP medium 2% glycerol, BMG buffered minimal medium 2% glycerol; Measurements were performed after 24 h of cultivation (BMM after 48 h). Values represent averages ± standard error (n = 4)
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us