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Table 1 Growth rates µ [h−1] of P. pastoris populations in different growth conditions

From: Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris

 

YPM

BMM

YPD

YPDN

BMD

BMDN

YPG

BMG

X-33

0.209 ± 0.001

0.089 ± 0.009

0.308 ± 0.002

0.281 ± 0.007

0.312 ± 0.005

0.264 ± 0.007

0.321 ± 0.008

0.269 ± 0.004

X-33 Y250 1

0.227 ± 0.002

0.108 ± 0.001

0.340 ± 0.004

0.278 ± 0.005

0.316 ± 0.007

0.269 ± 0.015

0.353 ± 0.006

0.250 ± 0.003

X-33 Y250 2

0.212 ± 0.006

0.123 ± 0.004

0.372 ± 0.002

0.282 ± 0.005

0.297 ± 0.008

0.205 ± 0.005

0.355 ± 0.007

0.265 ± 0.004

X-33 Y250 3

0.217 ± 0.005

0.142 ± 0.001

0.359 ± 0.005

0.279 ± 0.02

0.302 ± 0.005

0.316 ± 0.014

0.321 ± 0.004

0.285 ± 0.001

X-33 Y250 4

0.216 ± 0.003

0.136 ± 0.005

0.364 ± 0.004

0.291 ± 0.005

0.348 ± 0.011

0.356 ± 0.005

0.354 ± 0.004

0.260 ± 0.002

X-33 M250 1

0.192 ± 0.007

0.129 ± 0.002

0.334 ± 0.002

0.183 ± 0.003

0.286 ± 0.015

0.246 ± 0.017

0.327 ± 0.002

0.257 ± 0.007

X-33 M250 2

0.219 ± 0.003

0.127 ± 0.001

0.328 ± 0.002

0.260 ± 0.032

0.258 ± 0.009

0.240 ± 0.019

0.363 ± 0.002

0.310 ± 0.003

X-33 M250 3

0.199 ± 0.005

0.125 ± 0.007

0.368 ± 0.008

0.264 ± 0.006

0.259 ± 0.010

0.200 ± 0.013

0.350 ± 0.004

0.302 ± 0.011

X-33 M250 4

0.214 ± 0.001

0.126 ± 0.005

0.314 ± 0.03

0.270 ± 0.009

0.256 ± 0.004

0.215 ± 0.005

0.278 ± 0.013

0.289 ± 0.008

  1. Growth tests were performed in 24-deep well plates as described in the “Methods” section. X-33 ancestral strain, X-33 Y250a-d populations evolved on YPM medium, X-33 M250a-d populations evolved on BMM medium, YPM YP medium 1% MeOH, BMM buffered minimal medium 1% MeOH, YPD YP medium 2% glucose, YPDN YPD 500 mM NaCl, BMD buffered minimal medium 2% glucose, BMDN BMD 250 mM NaCl, YPG YP medium 2% glycerol, BMG buffered minimal medium 2% glycerol; values represent averages ± standard error (n = 4). Growth rates in italics differ significantly from X-33 ancestral growth rates (p < 0.05, Additional file 1: Table S3)