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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris

Fig. 2

AOX1 mutations and activity in several ancestral and evolved P. pastoris strains. a Aox1 protein domains according to the recently published crystal structure (PDB ID 5HSA) [49]. Amino acid positions mutated in the evolved strains are highlighted. b Alcohol oxidase activity in P. pastoris strains. Activity was determined as described in the “Methods” section. The enzymatic activity of wildtype P. pastoris X-33 on YPM and BMM growth medium was set to 100%. The activity of the wildtype strain on glucose and methanol is shown, as well as the activity of four evolved strains with mutations of the AOX1 gene on methanol as carbon source. YPM (black bars), BMM (grey bars); Values represent averages of two biological and two technical replicates ±standard deviation

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Microbial Cell Factories

ISSN: 1475-2859

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