Skip to main content

Table 1 Primers used in this study

From: Overexpression of a C4-dicarboxylate transporter is the key for rerouting citric acid to C4-dicarboxylic acid production in Aspergillus carbonarius

Name

Sequence (5′ → 3′)

Annotation

DctFw

AGAGCGAUATGCATGTCCACGACACC

SimpleUSER cloning of dct gene

DctRv

TCTGCGAUTCATTCAGACACATCCTCGTC

SimpleUSER cloning of dct gene

FrdFw

AGAGCGAUATGGTTGATGGTCGGTCGT

SimpleUSER cloning of frd gene

FrdRv

TCTGCGAUTTAGCTACCCGACGGTTCAGTT

SimpleUSER cloning of frd gene

GpdFw

GGCATTAAUTCGTGGACCTAGCTGATTCTG

PCR amplification of expression cassette

TrpRv

GGTCTTAAUTCGAGTGGAGATGTGGAGTG

PCR amplification of expression cassette

qDctFw

TTCCTTCCACCTCAACTGGT

qPCR dct gene

qDctRv

GCTGAGCAGGACAAAGATGA

qPCR dct gene

qActinFw

AGAGCGGTGGTATCCATGAG

qPCR beta-actin gene

qActinRv

TGGAAGAGGGAGCAAGAGCG

qPCR beta-actin gene