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Table 2 MVA gene RBS sequences with their calculated strength of representative strains from the combinatory expression library DXS37-IDI46 (pACYC184-AL-mva)

From: Type IIs restriction based combinatory modulation technique for metabolic pathway optimization

Strains The genes RBS of MVA pathway Relative yield (%)b
mvaE mvaS mvaK 1 mvaK 2 mvaD
Sequence Calculated strengtha Sequence Calculated strength Sequence calculated strength Sequence Calculated strength Sequence Calculated strength
ALV104 AGGAGGCTAGAA 17988 AGGAGAGTTTTA 16327 AGGAGAGTCTTT 12463 AGGAGGGGGTGC 30731 AGGAGATTCCTG 92 112.5
ALV131 AGGAGGCTTTGG 8370 AGGAGGTGGGGG 162464 AGGAGGTATTTC 183455 AGGAGGTTGCGT 90504 AGGAGAATGGGC 612 120.9
ALV100 AGGAGGGTTTGA 9158 AGGAGGGTGGGG 72267 AGGAGATTGTGC 7513 AGGAGGGTGTCC 29379 AGGAGAATGGGG 447 123.2
ALV108 AGGAGGTTGGTG 8110 AGGAGAAATTGG 2064 AGGAGGGGGGGT 35173 AGGAGGGGGGTT 15646 AGGAGAGTTTCG 259 129.6
ALV20 AGGAGGGGGCTA 15575 AGGAGACTTTGT 7615 AGGAGGGCGCCT 6080 AGGAGGTTTGCT 103587 AGGAGGTATGCT 3238 151.2
ALV63 AGGAGGAGGTGT 33777 AGGAGGCATGGG 44050 AGGAGAAACGGC 30183 AGGAGGGGTCTA 14690 AGGAGGTTGCAT 5813 163.9
ALV25 AGGAGACACAAT 10817 AGGAGGGCGGTT 15646 AGGAGGGCTTTT 25668 AGGAGGTGC 1258 AGGAGGCTTCGT 559 171.9
ALV133 AGGAGAGTTTAC 25607 AGGAGGACGAGG 17119 AGGAGGGCTCCT 22126 AGGAGGGCGGGC 12493 AGGAGATCGGCC 92 181.8
ALV23 AGGAGGGAGTAA 50645 AGGAGGTGGGTG 148479 AGGAGAGGGTCC 8332 AGGAGGGATGCG 11418 AGGAGGTGCCAT 1870 186.3
ALV145 AGGAGGGATTGT 33777 AGGAGGAGCTCT 57705 AGGAGGTGGTGT 160285 AGGAGGGGGTGC 30731 AGGAGGTCTTGC 1258 196.0
  1. aCalculated Strength was represented by the translation initiation rate calculated by RBS Library Calculator [9, 17]
  2. bRelative yield compared with their parent strain DXS37-IDI46