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Table 2 MVA gene RBS sequences with their calculated strength of representative strains from the combinatory expression library DXS37-IDI46 (pACYC184-AL-mva)

From: Type IIs restriction based combinatory modulation technique for metabolic pathway optimization

Strains

The genes RBS of MVA pathway

Relative yield (%)b

mvaE

mvaS

mvaK 1

mvaK 2

mvaD

Sequence

Calculated strengtha

Sequence

Calculated strength

Sequence

calculated strength

Sequence

Calculated strength

Sequence

Calculated strength

ALV104

AGGAGGCTAGAA

17988

AGGAGAGTTTTA

16327

AGGAGAGTCTTT

12463

AGGAGGGGGTGC

30731

AGGAGATTCCTG

92

112.5

ALV131

AGGAGGCTTTGG

8370

AGGAGGTGGGGG

162464

AGGAGGTATTTC

183455

AGGAGGTTGCGT

90504

AGGAGAATGGGC

612

120.9

ALV100

AGGAGGGTTTGA

9158

AGGAGGGTGGGG

72267

AGGAGATTGTGC

7513

AGGAGGGTGTCC

29379

AGGAGAATGGGG

447

123.2

ALV108

AGGAGGTTGGTG

8110

AGGAGAAATTGG

2064

AGGAGGGGGGGT

35173

AGGAGGGGGGTT

15646

AGGAGAGTTTCG

259

129.6

ALV20

AGGAGGGGGCTA

15575

AGGAGACTTTGT

7615

AGGAGGGCGCCT

6080

AGGAGGTTTGCT

103587

AGGAGGTATGCT

3238

151.2

ALV63

AGGAGGAGGTGT

33777

AGGAGGCATGGG

44050

AGGAGAAACGGC

30183

AGGAGGGGTCTA

14690

AGGAGGTTGCAT

5813

163.9

ALV25

AGGAGACACAAT

10817

AGGAGGGCGGTT

15646

AGGAGGGCTTTT

25668

AGGAGGTGC

1258

AGGAGGCTTCGT

559

171.9

ALV133

AGGAGAGTTTAC

25607

AGGAGGACGAGG

17119

AGGAGGGCTCCT

22126

AGGAGGGCGGGC

12493

AGGAGATCGGCC

92

181.8

ALV23

AGGAGGGAGTAA

50645

AGGAGGTGGGTG

148479

AGGAGAGGGTCC

8332

AGGAGGGATGCG

11418

AGGAGGTGCCAT

1870

186.3

ALV145

AGGAGGGATTGT

33777

AGGAGGAGCTCT

57705

AGGAGGTGGTGT

160285

AGGAGGGGGTGC

30731

AGGAGGTCTTGC

1258

196.0

  1. aCalculated Strength was represented by the translation initiation rate calculated by RBS Library Calculator [9, 17]
  2. bRelative yield compared with their parent strain DXS37-IDI46