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Table 1 Strains and plasmids in this study

From: Type IIs restriction based combinatory modulation technique for metabolic pathway optimization

Strains/plasmids

Relevant characteristics

Source/notes

Strains

 E. coli DH5α

F endA1thi-1 recA1 relA1 gyrA96deoRΦ80dlacΔ(lacZ) M15 Δ (lacZYA-argF) U169hsdR17 (r K , m +K ) λ supE44 phoA

Invitrogen

 CGMCC 1.2135

Enterococcus faecalis wild-type

CGMCC

 CGMCC 1.8722

Streptococcus Pneumoniae wild-type

CGMCC

 Dxs37-Idi46

ATCC 8739, ldhA::M1-12::crtEXYIB::ldhA, M1-37::dxs, M1-46::idi

Laboratory stock

Plasmids

 pACYC184-PgadA-RFP

E. coli expression vector derived from pACYC184, promoter of gadA, RFP, cat

Laboratory stock

 pACYC184-AL-mva

plasmid library of combinatorically regulated MVA pathway, derived from pACYC184-PgadA-RFP

This study