Skip to main content

Advertisement

Table 1 Strains and plasmids in this study

From: Type IIs restriction based combinatory modulation technique for metabolic pathway optimization

Strains/plasmids Relevant characteristics Source/notes
Strains
 E. coli DH5α F endA1thi-1 recA1 relA1 gyrA96deoRΦ80dlacΔ(lacZ) M15 Δ (lacZYA-argF) U169hsdR17 (r K , m K + ) λ supE44 phoA Invitrogen
 CGMCC 1.2135 Enterococcus faecalis wild-type CGMCC
 CGMCC 1.8722 Streptococcus Pneumoniae wild-type CGMCC
 Dxs37-Idi46 ATCC 8739, ldhA::M1-12::crtEXYIB::ldhA, M1-37::dxs, M1-46::idi Laboratory stock
Plasmids
 pACYC184-PgadA-RFP E. coli expression vector derived from pACYC184, promoter of gadA, RFP, cat Laboratory stock
 pACYC184-AL-mva plasmid library of combinatorically regulated MVA pathway, derived from pACYC184-PgadA-RFP This study