From: Type IIs restriction based combinatory modulation technique for metabolic pathway optimization
Strains/plasmids | Relevant characteristics | Source/notes |
---|---|---|
Strains | ||
E. coli DH5α | F− endA1thi-1 recA1 relA1 gyrA96deoRΦ80dlacΔ(lacZ) M15 Δ (lacZYA-argF) U169hsdR17 (r −K , m +K ) λ− supE44 phoA | Invitrogen |
CGMCC 1.2135 | Enterococcus faecalis wild-type | CGMCC |
CGMCC 1.8722 | Streptococcus Pneumoniae wild-type | CGMCC |
Dxs37-Idi46 | ATCC 8739, ldhA::M1-12::crtEXYIB::ldhA, M1-37::dxs, M1-46::idi | Laboratory stock |
Plasmids | ||
pACYC184-PgadA-RFP | E. coli expression vector derived from pACYC184, promoter of gadA, RFP, cat | Laboratory stock |
pACYC184-AL-mva | plasmid library of combinatorically regulated MVA pathway, derived from pACYC184-PgadA-RFP | This study |