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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Type IIs restriction based combinatory modulation technique for metabolic pathway optimization

Fig. 2

Modulation and optimization of MVA pathway with TRCM technique. a Primer design for obtaining assembly parts containing degenerate RBS sequences. Primers for amplification of MVA genes were embedded with BsaI recognition site, optimized four bp linkers, and random RBS sequence AGGAGRNNNNNN. b TRCM DNA parts for assembly. One ready-made vector part was used to assemble with five library parts. c Combinatory expression library of MVA pathway. The plasmid library contained all five genes regulated with degenerate RBSs in various combinations

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