Fig. 1From: Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae Relative activities of Erv1 and its variants. a GSH was used as a substrate; b γ-GC was used as a substrate. Erv1 and variants were assayed for 10 min at 30 °C and pH 7.0 (potassium phosphate buffer) using 10 mM substrates. The 100% relative activities of Erv1 using GSH and γ-GC as substrates were 1.62 and 1.59 mU mg−1, respectively. All assays were separately performed three times. Data are presented as the mean ± standard deviation (n = 3)Back to article page