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Table 2 Homologous residues of KstDs implicated in the catalytic pocket attending to Rohman et al. [21]

From: Functional differentiation of 3-ketosteroid Δ1-dehydrogenase isozymes in Rhodococcus ruber strain Chol-4

KstD1-SQ1

KstD1-ruber

KstD2-ruber

KstD3-ruber

S52

G53

A59

G50

F116

W118

Y125

Y116

Y119

Y121

Y128

Y119

F294

F295

P337

F327

V296

L297

V339

S330

Y318

Y319

Y366

Y354

I352

T356

 

I395

I354

V358

F405

P397

L447

L447

L499

L490

Y487

Y487

Y539

Y530

P490

G490

A542

P533

G491

G491

G543

G534

V492

N492

A544

A535

P493

P493

T545

T537

  1. Italic files contain the four key residues of the KstD1 active site reported for R. erythropolis SQ1: Tyr119, Tyr318, Tyr487 and Gly491