Fig. 4From: Functional differentiation of 3-ketosteroid Δ1-dehydrogenase isozymes in Rhodococcus ruber strain Chol-4ARF-TSS analysis of kstD transcripts. The translation initiation codon ATG, the TSS identified by ARF-TSS and the proposed −10 box appear in black. R1, R2 and F3 primers are indicated. A grey bar shows the sequence obtained by ARF-TSS from the circularized cDNA and the subsequent PCR amplification with R2 and F3Back to article page