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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Functional differentiation of 3-ketosteroid Δ1-dehydrogenase isozymes in Rhodococcus ruber strain Chol-4

Fig. 3

Comparative assessment of R. ruber kstD promoters. Cells of R. ruber strain Chol-4 harbouring different recombinant plasmids were grown in minimal medium supplemented with either 1.5 mM cholesterol (kstD2, kstD3 or kstD3 b promoters) or 2 mM AD (kstD1 promoter) in the presence of either 200 µg/mL kanamycin or 300 µg/mL apramycin. kstDp: R. ruber cells harbouring pNVSP plasmids (kstD promoters cloned in pNVS vector). kstDp- Amr: R. ruber cells harbouring pNVSP-A plasmids (apramycin resistance gene fused to kstD promoters in pNVSP plasmids). Amr: pNVS vector containing the promoter less apramycin resistance gene

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Microbial Cell Factories

ISSN: 1475-2859

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