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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Functional differentiation of 3-ketosteroid Δ1-dehydrogenase isozymes in Rhodococcus ruber strain Chol-4

Fig. 2

Sequence of regions upstream kstD ORFs. Solid arrows represent the orientation of the different ORFs from the initiation to the final codon. Sequences similar to Mycobacterium KstR binding sites (TnnAACnnGTTnnA) are within a square. Palindromic sequences appear in bold characters and dotted underlined. Shine Dalgarno (SD) sequences are in italics and underlined. The −10, −35 boxes are marked in grey. The in silico transcription initiation point of the putative pP1, pP2, pP3 and pP4 promoters are in italics and marked as pP(+1). The transcription start sites of kstD1 and kstD2 ORF obtained by the ARF-TSS method are shown in bold italics and marked as pKstD(+1). The TCCG repeats and the Sal box upstream kstD2 are double underlined. Promoter signals similar to others described (e.g. M. tuberculosis) [55] appear in bigger size. Primer CH488 indicating the beginning of the minimum kstD3 promoter is also shown

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