Fig. 5
Fps1 produced from plasmids containing 0, 1 or 2 uORFs rescues the osmosensitive phenotype of fps1Δ cells. Yeast cells (BY4741 (WT) or fps1Δ) were transformed with pYX222 (empty vector control), pYX222-FPS1-HA3 (containing 2 uORFs), pYX222-5′Δ1-43-FPS1-HA3 (containing 1 uORF) or pYX222-5′Δ1-215-FPS1-HA3 (containing 0 uORFs) and grown in 2× CBS (without histidine) supplemented with 1 M sorbitol. Cells were spotted (10 μL spots) in 10-fold serial dilutions onto YNB agar plates with or without 1 M sorbitol. A representative image is shown from three biologically independent determinations. Growth rates were calculated in 200 μL 2× CBS in a 96 well plate format (n = 6). Standard error of the mean (SEM) is given in parentheses. A one-way ANOVA followed by a Dunnett’s multiple comparison test was used to analyze the growth rate data where *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 and ****P ≤ 0.0001