Fig. 3

eIF2α phosphorylation is constitutive in high-yielding Fps1 strains. Phosphorylation of eIF2α, as detected by immunoblot, is shown in amino-acid-starved and control cells: (+) represents cells cultured in growth medium supplemented with amino acids (control cells) whilst (−) represents cells cultured in growth medium lacking amino acids for 10 min (starved cells). yTHCBMS1 cultures were also supplemented with 0.5 μg/mL doxycycline. The upper panel (37 kDa) was detected with an anti-eIF2α antibody, whilst the lower panel (37 kDa) was detected with an anti-phospho-eIF2α antibody