Fig. 1
Some high-yielding Fps1 strains exhibit initiation blocks. Representative polysome profiles for the high-yielding strains and the wild-type control. Cells were cultured in YPD medium and harvested at A600 ~ 1. Values in square brackets are the monosome/polysome ratio with the standard error of the mean shown in parentheses, as follows: a BY4741 wild-type control [0.15 (n = 3, 0.01)], b yTHCBMS1 [0.24 (n = 3, 0.03)], c yTHCBMS1 supplemented with 0.5 μg/mL doxycycline [0.43 (n = 3, 0.14)], d spt3Δ [0.18(n = 3, 0.03)], e srb5Δ [0.39 (n = 3, 0.07)], f gcn5Δ [0.16 (n = 2, 0.02)]