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Table 2 Shake flask PHB production by recombinants Halomonas sp. TD01 with controllable gltA gene expression via CRISPRi

From: Engineering Halomonas species TD01 for enhanced polyhydroxyalkanoates synthesis via CRISPRi

Recombinant TD01 strains

CDW (g/L)

PHB (wt%)

TD01

10.22 ± 0.25

77.68 ± 3.75

TD-sgRNA

13.28 ± 0.57

63.80 ± 3.08

TD-gltA1

13.68 ± 0.21

66.79 ± 1.59

TD-gltA2

13.53 ± 0.41

71.77 ± 7.16

TD-gltA3

13.18 ± 0.33

69.22 ± 3.43

TD-gltA4

13.10 ± 0.42

66.16 ± 8.11

  1. All strains were cultivated in MM medium containing 30 g/L glucose at 37 °C for 48 h as described in “Methods”. CDW, cell dry weight; PHBV (wt%), the weight percent of PHBV in CDW; TD01, Halomonas sp. TD wild type strain; TD-sgRNA, TD01 strain harboring the pli-dCas9-sgRNA plasmid without any DNA target site; TD-gltA1, TD-gltA2, TD-gltA3, TD-gltA4, TD01 strains harboring the pli-dCas9-sgRNA plasmid with different sgRNA targets of gene gltA, respectively. All data were the average of three independent studies with standard deviations. Mean ± SE (n = 3)
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Microbial Cell Factories

ISSN: 1475-2859

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