Fig. 4
From: Engineering Halomonas species TD01 for enhanced polyhydroxyalkanoates synthesis via CRISPRi
Controllable repression of prpC gene transcription in recombinant Halomonas sp. TD01. The relative binding positions of sgRNAs targeting prpC gene (a) and RT-PCR study of prpC transcription levels (b). All data were the average of three independent studies with standard deviations. Mean ± SE (n = 3). *p < 0.05 and **p < 0.01. TD01, Halomonas sp. TD wild type; TD-sgRNA, TD01 strain harboring the pli-dCas9-sgRNA plasmid without any target site; TD-prpC1, TD-prpC2, TD-prpC3, TD-prpC4, TD-prpC5, TD-prpC6, TD-prpC7, TD-prpC6prpC7, TD01 strains harboring the pli-dCas9-sgRNA plasmid with different DNA targets on gene prpC