Fig. 3
From: Engineering Halomonas species TD01 for enhanced polyhydroxyalkanoates synthesis via CRISPRi
Scanning electron microscopy study on Halomonas sp. TD01 with its ftsZ gene repressed via CRISPRi under 5000 (a) and 2000 (b) times magnification. TD01: wild type Halomonas sp. TD used as a control group; TD-sgRNA, TD01 strain harboring the pli-dCas9-sgRNA plasmid without any DNA target site; TD-ftsZ1, TD-ftsZ2, TD01 strain harboring the CRISPRi plasmids pli-dCas9-ftsZ1 and pli-dCas9-ftsZ2 that regulated the expression level of fission ring protein ftsZ gene, respectively. Scale bars are 50 or 20 µm, as indicated