Skip to main content

Table 3 Transition midpoint temperatures (T m) measured with the ThermoFAD method and the intrinsic tryptophan fluorescence

From: Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems

Enzyme

ThermoFAD

Trp unfolding

E. coli (DH)

57.7 ± 0.3

56.9 ± 0.4

P. pastoris (CDH)

59.2 ± 0.1

57.4 ± 0.6

A. niger (CDH)

59.7 ± 0.1

57.5 ± 0.5

T. reesei (CDH)

57.8 ± 0.2

56.6 ± 0.3

  1. The measurements were done in triplicates