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Table 2 Comparison of steady-state kinetic constants of CtDH and CtCDHs for cellobiose

From: Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems

Enzyme

KM (μM)

k cat (s−1)

k cat /KM (mM−1 s−1)

DCIP assay

 E. coli (DH)

367 ± 11

15.5 ± 0.2

42

 P. pastoris (CDH)

373 ± 14

8.1 ± 0.3

22

 A. niger (CDH)

357 ± 18

13.3 ± 0.3

37

 T. reesei (CDH)

349 ± 21

15.2 ± 0.4

44

Cyt c assay

 P. pastoris (CDH)

81 ± 7

2.6 ± 0.1

32

 A. niger (CDH)

102 ± 5

7.6 ± 0.2

74

 T. reesei (CDH)

118 ± 11

7.9 ± 0.2

67

  1. The measurements were done in triplicates