Skip to main content

Table 3 The relative expression levels of the particular genes in the parental strains and cat8Δ mutants at the third day of xylose alcoholic fermentation at 45 °C

From: Transcriptional activator Cat8 is involved in regulation of xylose alcoholic fermentation in the thermotolerant yeast Ogataea (Hansenula) polymorpha

ΔΔCt

Genes

XYL1

XYL2

XYL3

PDC1

TKL1

DAS1

TAL1

TAL2

RPE1

FBP1

PCK1

cat8Δ/WT

1.13 ± 0.300

1.05 ± 0.577

2.82 ± 0.438

0.64 ± 0.400

1.10 ± 0.361

2.39 ± 0.342

1.23 ± 0.360

1.21 ± 0.193

2.60 ± 0.486

0.67 ± 0.165

1.10 ± 0.435

BEP cat8Δ/BEP

0.36 ± 0.300

0.62 ± 0.085

1.07 ± 0.086

0.88 ± 0.479

0.79 ± 0.175

0.57 ± 0.195

1.11 ± 0.091

1.12 ± 0.067

1.54 ± 0.052

0.74 ± 0.392

0.76 ± 0.140

BEP/WT

7.66 ± 0.971

18.03 ± 0.045

2.76 ± 0.158

1.96 ± 0.380

1.71 ± 0.178

1.70 ± 0.670

9.29 ± 0.138

3.59 ± 0.138

47.60 ± 0.301

1.02 ± 0.274

0.19 ± 0.414

  1. The mRNA quantification was normalized to ACT1 mRNA
  2. Genes encode: XYL1, xylose reductase; XYL2, xylitol dehydrogenase; XYL3, xylulokinase; PDC1, pyruvate decarboxylase; TKL1, transketolase; DAS1, dihydroxyacetone phosphate synthase or peroxisomal transketolase; TAL1, transaldolase; TAL2, peroxisomal transaldolase; RPE1, ribulosephosphate epimerase; FBP1, fructose-1,6-bisphosphatase; PCK1, phosphoenolpyruvate carboxykinase
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us