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Table 3 The relative expression levels of the particular genes in the parental strains and cat8Δ mutants at the third day of xylose alcoholic fermentation at 45 °C

From: Transcriptional activator Cat8 is involved in regulation of xylose alcoholic fermentation in the thermotolerant yeast Ogataea (Hansenula) polymorpha

ΔΔCt Genes
XYL1 XYL2 XYL3 PDC1 TKL1 DAS1 TAL1 TAL2 RPE1 FBP1 PCK1
cat8Δ/WT 1.13 ± 0.300 1.05 ± 0.577 2.82 ± 0.438 0.64 ± 0.400 1.10 ± 0.361 2.39 ± 0.342 1.23 ± 0.360 1.21 ± 0.193 2.60 ± 0.486 0.67 ± 0.165 1.10 ± 0.435
BEP cat8Δ/BEP 0.36 ± 0.300 0.62 ± 0.085 1.07 ± 0.086 0.88 ± 0.479 0.79 ± 0.175 0.57 ± 0.195 1.11 ± 0.091 1.12 ± 0.067 1.54 ± 0.052 0.74 ± 0.392 0.76 ± 0.140
BEP/WT 7.66 ± 0.971 18.03 ± 0.045 2.76 ± 0.158 1.96 ± 0.380 1.71 ± 0.178 1.70 ± 0.670 9.29 ± 0.138 3.59 ± 0.138 47.60 ± 0.301 1.02 ± 0.274 0.19 ± 0.414
  1. The mRNA quantification was normalized to ACT1 mRNA
  2. Genes encode: XYL1, xylose reductase; XYL2, xylitol dehydrogenase; XYL3, xylulokinase; PDC1, pyruvate decarboxylase; TKL1, transketolase; DAS1, dihydroxyacetone phosphate synthase or peroxisomal transketolase; TAL1, transaldolase; TAL2, peroxisomal transaldolase; RPE1, ribulosephosphate epimerase; FBP1, fructose-1,6-bisphosphatase; PCK1, phosphoenolpyruvate carboxykinase