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Table 3 Membrane lipid content and composition changes in the wild type MG1655, ΔompF, ΔfadL, Pla-fadL strains

From: Improving Escherichia coli membrane integrity and fatty acid production by expression tuning of FadL and OmpF

Strain

Membrane lipid content (mg/g DCW)

Membrane lipid composition (mol %)

Membrane lipid length

Membrane lipid S/U ratio

C14:0

C16:1

C16:0

C17cyc

C18:1

C18:0

C19cyc

MG1655

69.4 ± 0.3

1.3 ± 0.1

13.6 ± 0.2

48.5 ± 0.2

14.1 ± 0.1

19.1 ± 0.4

1.70 ± 0.03

1.8 ± 0.1

16.4 ± 0.2

1.06 ± 0.02

ΔompF

71.3 ± 0.5 (+2.7%)

1.1 ± 0.1

13.6 ± 0.1

48.7 ± 0.1

13.6 ± 0.3

19.4 ± 0.1

1.9 ± 0.1

1.7 ± 0.1

16.4 ± 0.1

1.07 ± 0.01

ΔfadL

62 ± 3 (−10%)

1.2 ± 0.1

12.7 ± 0.1

48.3 ± 0.4

14.5 ± 0.1

19.4 ± 0.3

1.9 ± 0.1

1.9 ± 0.1

16.4 ± 0.2

1.06 ± 0.01

Pla-fadL

78 ± 1 (+13%)

1.3 ± 0.1

13.2 ± 0.2

48.2 ± 0.1

13.4 ± 0.1

20.7 ± 0.1

1.9 ± 0.2

1.2 ± 0.1

16.4 ± 0.1

1.00 ± 0.02

  1. Each value is an average and standard deviation of three biological replicates
  2. All experiments were performed in MOPS + 2% (wt/v) dextrose shake flasks at 220 rpm 30 °C with an initial pH of 7.0, 10 mM octanoic acid (C8). All values are the average of at least three biological replicates with the associated standard deviation indicated. Percent increase values are only shown for differences that were deemed statistically significant (P < 0.05)
  3. DCW dry cell weight, S/U ratio membrane saturated: unsaturated lipid ratio