Table 1 Properties of promoters used for β-CGTase expression optimization
Promoter | Origin | Properties | Expression reporter proteins |
|---|---|---|---|
Psrf | B. subtilis | Auto-inducible system regulated by ComA–ComP phosphorylation system [14] | Green florescent protein, aminopeptidase |
Pxyl′ | B. subtilis | Xylose-based expression system and catabolite repressed by catabolite-responsive element [11] | β-Galactosidase, glycerol-3-phosphate cytidylyltransferase |
PgsiB | B. subtilis | Subject to σB regulation and is induced by ethanol, heat and acid shock [30] | β-Galactosidase [50] |
Pxyl | B. megaterium | Xylose-based expression system and glucose repression [10] | β-Galactosidase and other heterologous proteins |
PHpaII | Staphylococcus aureus | Strong constitutive promoter that stimulates counterclockwise RNA synthesis [13] | β-Galactosidase, chloramphenicol acetyltransferase and other heterologous proteins |
PamyQ′ | B. subtilis | Regulated by the DegS–DegU two-component system [32] | β-Galactosidase |
PaprE | B. subtilis | Promoter of alkaline protease | None |
PnprE | B. subtilis | Promoter of neutral protease | None |