Fig. 1

Schematic representation of plasmid construction. a Representation of the expression vector. The zeta region allows random insertion in the Y. lipolytica genome. LoxP and LoxR were used to rescue the marker. b Representation of the hybrid promoters containing pTEF (in gray), the UAS1 tandem elements (in brown), and the TATA box of the LEU2 promoter (in red). c Representation of the genes of interest (in various shades of blue); the preLip2 secretion sequence was present (in yellow) or absent