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Table 1 Kinetic analysis of the purified recombinant COMT

From: De novo biosynthesis of pterostilbene in an Escherichia coli strain using a new resveratrol O-methyltransferase from Arabidopsis

Substrates K m [μM] K cat [s−1] Catalytic efficiency K cat /K m
Caffeic acid 40.5 ± 6.6 (35.3 ± 5.7) × 10−3 872.8
Resveratrol 44.9 ± 3.2 (12.8 ± 1.4) × 10−3 283.9
  1. Determination of the K m and K cat of the COMT activity for caffeic acid to ferulic acid and ROMT activity for resveratrol to pinostilbene. Purified COMT (4 μg) was incubated with different concentrations of substrate for 30 min at 37 °C. The assay conditions are described in the “Methods” section. The data represent the mean S.D. of triplicate experiments